Core-2 O-glycans are required for galectin-3 interaction with synovial lubricin

PubMed Entry

GlyToucan Accession Number: G40269CS

Peak Intensity
188.93 0.2
189.95 1.4
190.56 0.0
190.88 0.2
191.41 0.2
192.0 5.6
192.99 1.0
194.04 30.1
194.89 4.1
196.0 5.0
197.09 0.2
198.07 4.1
198.65 0.0
199.19 0.4
200.05 7.2
200.86 3.3
201.91 11.9
202.17 6.8
202.53 0.6
202.79 0.9

Annotations:

^{2,4}A_{GalNAc}Z
203.29 1.1

Annotations:

^{2,4}A_{GalNAc}Z
204.02 196.8

Annotations:

Z
205.07 4.3
205.84 2.7
206.08 1.1
206.79 0.5
207.99 26.4
209.02 9.1
210.01 12.6
210.86 0.6
211.1 0.5
211.97 27.3
212.95 13.4
213.82 2.7
214.38 0.0
214.76 0.3
215.6 0.3
215.97 2.5
216.17 2.4
216.64 0.8
216.86 0.7
218.04 3.6
218.52 3.4
219.1 1.9
219.97 29.7
221.09 0.8

Annotations:

^{2,4}A_{GalNAc}Y
222.06 133.2

Annotations:

Y
223.1 3.8
223.97 5.0
226.06 17.7
226.46 2.2
227.0 1.1
227.36 1.1
227.98 6.4
228.55 0.0
229.42 2.9
229.98 22.4
230.96 3.8
231.33 0.2
231.81 0.8
232.13 0.6
232.87 0.5
233.15 0.4
234.18 2.7
234.74 0.1
235.29 0.0
235.98 4.9
236.93 1.2
237.21 3.4
238.07 3.2
239.02 0.8
239.84 1.2
240.47 0.0
241.16 0.2
241.99 2.8
242.61 0.0
242.99 0.1
243.69 1.0

Annotations:

^{1,3}A_{GalNAc}Z
244.08 4.2

Annotations:

^{1,3}A_{GalNAc}Z
245.28 1.7
245.97 10.9
246.99 0.7

Annotations:

^{2,5}A_{GalNAc}Z
248.07 2.7
248.92 0.3
250.08 2.9
250.8 0.4
252.01 6.2
253.17 5.5
253.88 12.9
254.72 0.3
255.27 0.0
256.0 1.7
256.67 0.0
257.17 0.0
258.12 0.4
258.33 0.2
258.69 0.0
258.91 0.0
260.12 3.0
260.69 0.0
261.08 0.6
262.08 4.1

Annotations:

^{1,3}A_{GalNAc}Y
262.84 1.7

Annotations:

^{0,2}A_{GalNAc}Z
263.27 0.8

Annotations:

^{0,2}A_{GalNAc}Z
263.88 7.2
264.5 0.0
264.85 0.5

Annotations:

^{2,5}A_{GalNAc}Y
265.41 0.0

Annotations:

^{2,5}A_{GalNAc}Y
265.95 0.0
266.48 0.0
267.03 0.0
267.78 8.7
268.09 9.6
268.71 0.0
269.24 1.1
270.05 26.2
270.96 11.5
271.99 212.6
272.9 9.7
273.18 4.1
273.73 2.5

Annotations:

^{1,4}A_{GalNAc}Z
274.09 2.6

Annotations:

^{1,4}A_{GalNAc}Z
274.72 0.2
275.27 0.0
275.89 22.6
276.2 14.4
277.14 0.7
277.84 0.4
278.1 0.3
279.06 0.3
279.63 0.0
280.19 0.0
280.48 0.1
280.86 2.5

Annotations:

^{0,2}A_{GalNAc}Y
281.61 0.2
282.03 0.4
282.86 0.2
283.43 0.0
283.97 0.2
284.53 0.0
285.05 0.0
285.7 0.9
286.03 1.7
286.23 0.9
287.94 99.5
289.01 44.5
289.98 23213.9

Annotations:

B
290.96 1549.1
291.98 34.3

Annotations:

^{1,4}A_{GalNAc}Y
292.46 1.1

Annotations:

^{1,4}A_{GalNAc}Y
293.18 0.5
293.78 0.2
294.36 0.0
294.85 0.1
295.41 0.0
295.84 0.6
296.07 0.2
296.94 1.3
297.18 0.3
298.17 2.2
298.73 0.0
299.28 0.0
299.75 0.6
300.19 2.4
300.77 0.0
301.22 0.3
301.56 0.5
302.09 7.1
302.68 0.0
303.21 0.0
303.75 0.6
304.13 0.5
304.74 0.0
305.28 0.0
305.87 3.9
306.15 6.5
306.98 0.8
307.27 1.7
307.93 58.2

Annotations:

C
309.04 18.0
309.88 1.5
310.53 0.0
311.07 0.0
311.93 4.8
313.38 0.3
314.01 0.4
314.35 0.0
314.59 0.0
314.96 0.3
315.26 0.1
315.8 0.8
316.03 1.0
316.29 0.6
316.76 0.4
317.13 0.6
317.79 0.7

Annotations:

^{1,5}A_{GalNAc}Z
318.12 9.6

Annotations:

^{1,5}A_{GalNAc}Z
318.52 2.1

Annotations:

^{1,5}A_{GalNAc}Z
319.47 0.7
319.99 7.4
320.58 0.0
321.12 0.0
321.77 0.6
322.07 0.4
322.36 0.4
323.14 1.2
324.03 15.3
324.98 2.4
325.94 4.3
326.54 0.0
327.09 0.0
328.1 1.5
328.88 2.1
329.13 1.1
329.86 0.4
330.32 0.2
330.79 0.3
331.31 0.9
331.97 24.2
332.94 0.2
333.45 3.4
334.06 6.7
334.35 3.3
335.0 0.7
335.8 0.7

Annotations:

^{1,5}A_{GalNAc}Y
336.08 1.3

Annotations:

^{1,5}A_{GalNAc}Y
336.91 0.0
337.97 0.5
338.6 0.0
339.15 0.0
339.86 0.5
340.08 0.2
340.61 0.0
340.94 0.0
341.18 0.0
341.52 0.3
341.85 2.0
342.26 2.7
342.9 0.6
343.45 0.0
344.0 4.9
344.97 0.3
345.94 13.8
346.64 0.2
347.12 1.4
347.87 0.8
348.24 0.7
349.09 0.2
349.63 0.5

Annotations:

^{1,3}A_{Gal} ^{2,4}A_{Gal}
350.05 1.6

Annotations:

^{1,3}A_{Gal} ^{2,4}A_{Gal}
350.63 0.0
351.19 0.0
351.64 0.4
352.17 0.2
352.72 0.0
353.26 0.0
353.83 0.0
354.34 0.0
355.0 0.3
355.72 0.3
356.28 0.0
356.8 0.0
357.44 0.5
358.17 3.8
358.99 7.0
359.54 0.0
360.16 0.5
360.53 0.0
361.08 0.3
362.15 3.3
362.95 0.1
364.04 2.4
364.85 0.5
365.13 0.3
366.06 13.8

Annotations:

Z
366.63 0.0

Annotations:

Z
367.15 0.7
368.11 1.3
368.66 0.0
369.21 0.0
370.01 2.5
370.25 1.1
371.15 0.4
372.05 1.4
372.55 0.0
372.75 0.0
373.25 0.0
373.94 8.0
374.35 0.3
374.6 0.2
375.14 0.0
375.54 0.3
375.93 5.0
376.17 2.3
376.77 0.4
377.17 0.2
377.83 0.7
378.39 0.0
378.91 0.0
379.96 260.4

Annotations:

^{1,4}A_{Gal}
381.0 25.5
381.77 2.8
382.32 0.8
383.03 1.9
384.01 800.1

Annotations:

Y
385.04 49.4
386.01 41.3
387.02 1.8
387.99 11.3
388.91 0.5
389.24 0.2
389.72 0.4
390.11 1.0
390.33 0.4
390.68 0.0
390.91 0.0
391.41 0.0
392.28 0.3
392.61 0.0
393.0 0.1
393.55 0.0
393.95 1.1

Annotations:

^{2,5}A_{Gal}
394.67 0.0
395.01 0.0
395.22 0.0
395.74 0.7
396.26 0.4
396.8 0.0
397.33 0.6
397.88 12.5
398.18 5.1
398.62 3.0
399.05 6.1
399.62 0.0
400.12 0.3
400.69 0.0
401.21 0.4
401.77 0.0
402.38 0.3
402.94 0.0
403.96 60.8
404.95 1.3
406.07 28.2
406.76 7.0
407.27 1.0
407.99 53.4
408.7 0.7
409.07 5.7
410.11 8.0

Annotations:

^{0,2}A_{Gal}
410.72 0.0
411.2 0.2
411.88 1.2
412.43 0.0
412.95 0.0
413.63 0.3
414.18 0.4
415.02 0.2
415.41 0.8
415.98 47.2
416.95 3.1
417.28 2.1
417.91 0.6
418.17 0.2
418.75 0.0
419.26 0.0
419.81 0.0
420.34 0.0
421.2 0.2
422.13 1.5
422.85 1.0
423.32 0.2
424.06 0.5

Annotations:

^{1,5}A_{Gal}
424.48 0.3

Annotations:

^{1,5}A_{Gal}
424.94 0.3
425.4 0.6
426.14 0.2
426.69 0.0
427.21 0.0
427.77 0.0
428.28 0.0
428.97 0.4
429.18 0.7
429.72 0.0
430.27 0.0
430.79 0.0
431.11 0.0
431.36 0.0
431.85 0.3
432.4 0.0
432.87 0.1
433.12 0.2
434.0 47.7
434.71 0.7
435.03 2.4
435.43 0.2
435.99 4.8
436.69 0.0
436.9 0.0
437.27 0.0
437.94 0.3
438.2 0.6
438.87 0.4
439.5 0.0
439.93 0.0
440.58 0.0
440.92 0.3
441.47 0.0
442.02 0.0
442.84 0.4
443.39 0.0
443.94 0.0
444.46 0.0
445.02 0.0
445.54 0.0
446.1 0.0
446.62 0.0
447.11 0.2
447.65 0.0
448.19 0.0
449.01 0.3
449.58 0.0
450.07 0.8
451.03 2.9
452.05 7.1

Annotations:

B
453.05 3.4
454.02 203.6
454.99 16.5
455.26 10.6
455.84 0.0
456.37 0.0
456.92 0.0
457.44 0.0
458.07 0.2
458.73 0.1
459.27 0.0
459.79 0.0
460.35 0.0
460.87 0.0
461.44 0.0
461.95 0.0
462.83 1.1
463.2 0.2
464.04 0.1
464.59 0.0
465.11 0.0
465.63 0.1
466.06 1.0
466.6 0.0
467.13 0.0
467.65 0.1
468.09 0.4
468.32 0.1
468.88 1.7
469.99 357.8

Annotations:

C
471.02 197.7
472.01 43.3
473.07 0.5
473.64 0.0
474.12 0.4
474.82 0.6
475.36 0.0
475.9 0.0
476.48 1.5
476.95 39.0
477.18 28.7
477.93 3.8
478.28 2.5
478.82 0.0
479.37 0.0
479.89 0.0
480.71 0.3
481.15 0.8
481.77 0.2
482.05 0.6
483.05 0.9
483.88 2.9
484.28 0.2
484.85 0.0
485.22 0.1
485.9 0.4
486.39 0.3
486.94 0.0
487.47 0.0
488.02 0.0
488.54 0.0
488.91 0.8
490.09 2.6
490.41 0.3
491.33 0.2
491.88 0.0
492.59 0.3
493.1 1.3
493.65 0.0
494.26 0.2
494.71 0.3
495.04 0.6
495.62 0.0
496.13 0.0
496.82 0.9
497.12 0.5
497.69 0.0
498.2 0.0
498.75 0.0
499.28 0.0
499.91 0.1
500.46 0.0
501.02 0.0
501.54 0.0
502.1 0.0
502.62 0.0
503.16 0.0
503.7 0.0
504.23 0.0
506.92 5.7
507.19 6.3
507.92 0.8
508.2 0.6
508.76 0.0
509.28 0.0
509.94 2.4
510.9 1.1
511.36 0.2
512.0 0.5

Annotations:

^{2,4}A_{GalNAc}
513.13 8.6
513.47 2.1
514.02 6.1
515.3 0.3
516.06 0.1
516.61 0.0
517.13 0.0
517.69 0.0
518.21 0.0
518.89 0.4
519.29 0.0
519.51 0.0
519.88 0.3
520.75 0.1
521.3 0.0
521.86 0.0
523.1 6.0
523.48 0.5
523.83 0.5
524.38 0.0
524.94 0.0
525.46 0.0
526.02 0.0
526.54 0.0
527.1 0.0
527.62 0.0
528.16 0.0
528.69 0.0
530.99 2.7
531.56 0.0
532.12 0.0
532.64 0.0
533.2 0.0
533.72 0.0
534.27 0.0
534.8 0.0
535.34 0.0
535.88 1.6
536.16 0.7
536.46 0.3
537.19 3.1
537.76 0.0
538.14 0.3
538.71 0.0
539.02 0.1
539.31 0.6
540.15 0.1
540.98 1.3
541.22 0.2
541.77 0.0
542.32 0.0
542.85 0.0
543.39 0.0
543.93 0.0
544.43 0.0
544.76 0.0
545.02 0.0
545.51 0.0
545.82 0.0
546.1 0.0
548.69 0.3
549.23 1.0
549.96 0.3
550.73 0.3
551.28 0.0
551.81 0.0
552.36 0.0
552.89 5.6

Annotations:

^{1,3}A_{GalNAc}
553.23 0.3

Annotations:

^{1,3}A_{GalNAc}
553.9 4.6
554.2 2.4
555.06 1.1
555.82 0.1

Annotations:

^{2,5}A_{GalNAc}
556.36 0.0

Annotations:

^{2,5}A_{GalNAc}
556.9 0.0
557.44 0.0
557.97 0.0
558.53 0.0
558.93 0.2
559.48 0.0
560.03 0.0
560.55 0.0
560.88 0.4
561.17 0.9
561.71 0.0
562.26 0.0
562.78 0.0
563.22 0.1
563.78 0.0
564.32 0.0
564.86 0.0
565.39 0.0
565.99 0.1
566.38 1.9
566.9 22.4
567.2 17.4
568.1 12.7
568.75 0.4
569.29 0.0
569.85 0.0
570.36 0.0
570.83 5.7
571.16 4.9
571.7 0.2

Annotations:

^{0,2}A_{GalNAc}
572.12 0.1

Annotations:

^{0,2}A_{GalNAc}
572.69 0.2
573.24 0.0
574.05 0.3
574.61 0.0
575.14 0.0
575.69 0.0
576.21 0.0
576.77 0.8
577.13 1.5
577.63 0.1
577.91 0.7
578.29 2.5
578.94 3.0
579.23 1.0
579.86 0.0
580.42 0.0
581.08 1.0
581.61 0.0
582.07 0.1
582.63 0.0
583.01 0.6

Annotations:

^{1,4}A_{GalNAc}
583.56 0.0

Annotations:

^{1,4}A_{GalNAc}
584.09 0.0
585.12 3.1
585.68 0.0
585.99 0.3
586.54 0.0
587.0 0.1
587.55 0.0
588.11 0.0
589.19 1.3
589.92 0.4
590.46 0.0
591.1 2.7
591.81 0.2
592.2 0.4
592.77 0.0
593.28 0.0
593.83 0.0
594.46 0.8
594.78 1.6
595.25 0.7
595.62 1.5
596.02 1.6
597.03 16.9
597.77 2.3
598.06 3.1
598.36 0.5
598.87 0.3
599.44 0.0
600.05 0.2
600.61 0.0
601.13 0.0
601.69 0.0
602.03 0.3
602.86 0.5
603.44 0.0
603.96 0.0
604.52 0.0
604.9 0.1
605.44 0.0
605.97 0.0
606.53 0.0
607.05 0.0
607.61 0.0
608.13 0.0
608.55 0.4
608.97 2.5
610.0 1.7
610.27 0.6
611.0 1.6
611.54 0.0
612.11 0.0
613.01 103.0
614.04 84.8
615.0 46.9
616.11 3.3
616.41 0.5
616.91 5.2
617.46 0.0
618.02 0.0
618.49 0.2
619.05 0.3
619.53 0.2
620.1 0.0
621.18 0.3
621.76 0.0
622.12 0.0
622.33 0.0
622.74 2.4
623.28 0.0
623.84 0.0
624.36 0.0
624.9 0.0
625.66 0.3
625.99 0.2
626.91 5.0

Annotations:

^{1,5}A_{GalNAc}
627.27 0.5

Annotations:

^{1,5}A_{GalNAc}
628.09 2.4
629.16 1.3
629.54 0.0
629.73 0.0
630.4 1.1
631.07 212.1
632.21 10.4
632.94 0.6
633.24 0.9
633.87 0.2
634.42 0.0
634.95 0.0
635.48 0.0
636.03 0.0
636.84 0.2
637.26 0.9
637.67 0.1
639.1 8.3
639.95 2.1
641.16 3.0
641.72 0.0
642.27 0.0
643.16 0.6
643.89 0.7
644.36 0.2
644.94 0.0
645.45 0.0
646.0 0.0
646.53 0.0
647.07 0.0
647.61 0.0
648.14 0.0
648.69 0.0
649.2 0.0
675.1 0.3

Annotations:

675.66 0.0

Annotations:

676.2 0.0
676.73 0.0
677.28 0.0
677.8 0.0
678.36 0.0
678.88 0.0
679.44 0.0
679.95 0.0

There are 24 similar spectra:

Id Structure Score

5225

1.00

4233

1.00

3747

1.00

4802

1.00

4203

1.00

4079

1.00

3872

1.00

3493

1.00

3637

1.00

5198

1.00

3972

0.99

5188

0.99

3636

0.95

5182

0.94

5224

0.92

4232

0.92

3746

0.91

4078

0.91

4202

0.91

3492

0.91

4801

0.90

3113

0.89

3871

0.89

3971

0.74
Report errors in this entry