N-glycans and O-glycans released from purified mammalian glycoprotein standards and proteins from cell lysate

PubMed Entry

Peak Intensity
289.9776449 5.2982974052

Annotations:

B
368.1599979 1.4408693314

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Y
424.2194155 4.754879117

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BYY BY CYZ CZY CZ
455.319996 1.2869049311

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YYY
553.2399938 1.3894456625

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Z
587.119993 1.0350933075

Annotations:

^{0,2}A_{GlcNAc}YZ ^{0,2}A_{GlcNAc}ZY ^{1,3}X_{GlcNAc}YY ^{1,3}X_{GlcNAc}YY YYY
596.214797 3.0063626766

Annotations:

BZ^{3,5}X_{Gal} BZ^{3,5}X_{Man} B^{0,2}X_{NeuAc} ^{2,5}A_{GlcNAc}YZ ^{2,5}A_{GlcNAc}ZY ^{1,3}X_{GlcNAc}ZZ ^{1,3}X_{GlcNAc}ZZ ^{3,5}X_{NeuAc}ZZ
652.3401497 2.1864165068

Annotations:

C^{1,5}X_{NeuAc} BZZ BZZ BZZ BZZ ^{0,3}A_{GlcNAc}Y
655.4068198 6.7376253605

Annotations:

B
693.3583471 5.7666941881

Annotations:

BZZ BZZ YZ ZY
701.8799905 1.3819966316

Annotations:

Z
715.4214493 3.2067590952

Annotations:

YZ ZY
730.3817357 2.9531680346

Annotations:

YYZ YZY ZYY YZ ZY
751.1898552 7.911524415

Annotations:

^{0,2}A_{GlcNAc}ZZ B^{1,3}X_{NeuAc}Y C^{1,3}X_{NeuAc}Z ^{1,5}A_{GlcNAc}Y Y^{0,2}X_{Gal}Z YZ^{0,2}X_{Fuc} Y^{0,2}X_{Man}Z ZY^{0,2}X_{Fuc} ^{0,2}X_{Gal}Z Z^{0,2}X_{Fuc} ^{0,2}X_{Man}YZ ^{0,2}X_{Man}ZY ^{2,5}X_{Man}Z Y^{2,5}X_{Man}Z Z^{0,2}X_{Gal}Y Z^{0,2}X_{Man}Y Z^{2,5}X_{Man}Y ^{2,5}X_{Gal}Z Z^{2,5}X_{Gal} Z^{2,5}X_{Man} ^{2,5}X_{Man}YZ ^{2,5}X_{Man}ZY
774.8574016 2.3945724964

Annotations:

Y
782.8684612 35.3152770996

Annotations:

YY
783.4129015 10.4294623137

Annotations:

YY
817.403926 7.1365445852

Annotations:

B
818.3999879 1.0205913782

Annotations:

^{0,3}A_{GlcNAc}YY ^{1,4}A_{GlcNAc} C^{0,3}X_{Gal} C^{1,4}X_{Gal} C^{0,3}X_{Man} C^{0,3}X_{Gal} C^{0,3}X_{Man} Z^{3,5}X_{Fuc} ^{1,4}X_{NeuAc}YZ ^{1,4}X_{NeuAc}ZY ^{3,5}A_{GlcNAc}Y
835.3821684 83.6914744377

Annotations:

C
836.3665375 15.9140998125

Annotations:

^{0,4}X_{Man}ZZ ^{2,4}X_{Man}ZZ ^{0,4}X_{Gal}ZZ ^{0,4}X_{Man}ZZ ^{2,4}X_{Man}ZZ ^{0,4}X_{GlcNAc}ZZ
851.4143634 3.0458645821

Annotations:

^{0,4}X_{GlcNAc}YY Y^{1,3}X_{Man}Y YY^{0,4}X_{Fuc} ^{1,3}X_{Man}YY YY^{0,4}X_{Fuc} ^{1,5}A_{GlcNAc}YZ ^{1,5}A_{GlcNAc}ZY
856.359987 1.4118386507

Annotations:

Y
866.9852607 15.5404266119

Annotations:

ZZ
884.4450619 6.7480603456

Annotations:

Z
908.4580926 4.987811327

Annotations:

^{1,4}X_{NeuAc}Y ^{3,5}A_{GlcNAc}YY
910.4399858 1.2309584618

Annotations:

^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}Y
926.9289165 14.5962029696

Annotations:

^{0,4}X_{Gal}Z ^{0,4}X_{Man}Z ^{2,4}X_{Man}Z ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Fuc} ^{0,4}X_{Man}Z ^{2,4}X_{Man}Z ^{1,3}X_{NeuAc}Z ^{0,4}X_{GlcNAc}Z
935.9852784 9.3005815744

Annotations:

^{0,4}X_{Gal}Y ^{0,4}X_{Man}Y ^{2,4}X_{Man}Y ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{0,4}X_{Fuc} ^{0,4}X_{Man}Y ^{2,4}X_{Man}Y ^{1,3}X_{NeuAc}Y ^{0,4}X_{GlcNAc}Y ^{0,4}X_{GlcNAc} B^{2,5}X_{NeuAc}Y C^{2,5}X_{NeuAc}Z ^{0,2}A_{GlcNAc}YZ ^{0,2}A_{GlcNAc}ZY ^{2,5}A_{GlcNAc}YZ ^{2,5}A_{GlcNAc}ZY ^{2,5}A_{GlcNAc}Z ^{2,5}X_{NeuAc}
958.1999847 1.2748826742

Annotations:

Y
980.780343 2.0428931713

Annotations:

^{1,5}X_{Gal}YY Y^{1,5}X_{Man}Y YY^{1,5}X_{Fuc} ^{1,5}X_{Man}YY Y^{1,5}X_{Gal}Y YY^{1,5}X_{Fuc}
1003.379903 4.0665874481

Annotations:

B^{0,2}X_{Man}Z B^{0,4}X_{NeuAc}Z B^{0,2}X_{Man}Z
1008.997287 12.0510551929

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{0,4}X_{Man} ^{2,4}X_{Man} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{0,4}X_{Man} ^{2,4}X_{Man} ^{0,4}X_{GlcNAc} C^{0,3}X_{NeuAc}Y ^{1,5}A_{GlcNAc}YY
1095.540034 3.4827424288

Annotations:

^{2,4}A_{GlcNAc}YZ ^{2,4}A_{GlcNAc}ZY ^{2,4}A_{GlcNAc}Z ^{2,4}A_{GlcNAc}YZ ^{2,4}A_{GlcNAc}ZY ^{2,4}A_{GlcNAc}Z BY^{0,2}X_{Gal} BY^{0,2}X_{Man} B^{0,2}X_{Gal} B^{0,2}X_{Man}Y C^{0,2}X_{Man}Z CZ^{0,2}X_{Gal} CZ^{0,2}X_{Man} ^{1,5}A_{Man}Z
1183.55998 1.0490365028

Annotations:

^{2,4}A_{GlcNAc}Z
1216.631252 5.5211997032

Annotations:

^{0,4}X_{Man}YY ^{1,3}X_{Man}Y ^{2,4}X_{Man}YY ^{0,4}X_{GlcNAc}YY Y^{1,3}X_{Man}Y YY^{0,4}X_{Fuc} ^{1,3}X_{Gal}YY ^{2,4}X_{Gal}YY YY^{0,4}X_{Fuc} Y^{0,4}X_{Gal}Y Y^{1,3}X_{Gal}Y Y^{2,4}X_{Gal}Y YY^{0,4}X_{Fuc} Y^{0,4}X_{Man}Y Y^{2,4}X_{Man}Y Y^{0,4}X_{Fuc} ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y
1233.501109 3.7957048416

Annotations:

CY
1261.578722 7.0979328156

Annotations:

^{0,2}A_{GlcNAc}Y C^{2,4}X_{NeuAc}Y C^{1,3}X_{GlcNAc}Y C^{2,4}X_{NeuAc}Y
1275.520479 14.1441001892

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^{2,4}A_{GlcNAc}Y
1327.700381 2.1612560749

Annotations:

^{0,4}X_{Gal}YZ Y^{1,5}X_{Gal}Z Z^{1,5}X_{Gal}Y ^{0,4}X_{Man}YZ ^{2,4}X_{Man}YZ ^{0,4}X_{Man}Z ^{0,4}X_{Gal}ZY ^{1,5}X_{Gal}YZ ^{1,5}X_{Gal}ZY YZ^{1,5}X_{Fuc} ZY^{1,5}X_{Fuc} ^{0,4}X_{Man}ZY ^{2,4}X_{Man}ZY ^{0,4}X_{GlcNAc}YZ ^{0,4}X_{GlcNAc}ZY ^{0,4}X_{GlcNAc}Z
1344.575531 5.6030660868

Annotations:

BY BY CZ CZ
1362.654075 14.3688259125

Annotations:

CY CY
1363.644339 5.681674242
1370.639976 1.0827156305
1386.696892 4.1724197865

Annotations:

ZZ
1419.503798 2.5572705269

Annotations:

^{1,3}X_{Gal}YY ^{2,4}X_{Gal}YY Y^{0,4}X_{Gal}Y Y^{1,3}X_{Gal}Y Y^{2,4}X_{Gal}Y YY^{0,4}X_{Fuc} Y^{0,4}X_{Man}Y Y^{2,4}X_{Man}Y ^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y
1422.698663 6.9888423681

Annotations:

YY Y
1423.879974 1.0030078888

Annotations:

C^{2,4}X_{NeuAc}
1443.664378 2.5757457018

Annotations:

YZZ ZYZ ZZY ZZ
1505.590356 6.1613754034

Annotations:

C^{0,4}X_{Man}Y C^{2,4}X_{Man}Y C^{0,4}X_{Gal}Y C^{0,4}X_{Man}Y C^{2,4}X_{Man}Y C^{0,4}X_{GlcNAc}Y ^{1,3}X_{NeuAc}YZ ^{1,3}X_{NeuAc}ZY Y^{0,2}X_{Man}Y Y^{0,2}X_{Gal} Y^{0,2}X_{Man} ^{0,2}X_{Man}Y ^{0,2}X_{Gal}Y ^{0,2}X_{Man}YY
1506.758322 27.1571184397

Annotations:

B
1507.667857 12.1106222868

Annotations:

Y^{1,5}X_{Gal}Y ^{1,5}X_{Gal}Y ^{0,4}X_{Man}YY ^{1,3}X_{Man}Y ^{2,4}X_{Man}YY Y^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Y YY^{1,5}X_{Fuc} ^{0,4}X_{Gal}YY Y^{1,5}X_{Fuc} ^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y
1524.676065 69.7634342909

Annotations:

C
1525.604953 15.5522633791
1548.935632 3.1988395452
1583.839971 1.1702196598
1607.681703 7.1309025288

Annotations:

Z
1608.776763 20.1572350264

Annotations:

B^{2,4}X_{NeuAc} B^{1,3}X_{GlcNAc}
1609.83997 1.0522041321

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Y^{0,2}X_{Fuc} ^{2,5}X_{Man}Y ^{2,5}X_{Man}Y
1623.770241 5.8873972893

Annotations:

YZ ZY
1624.619983 2.2612538338
1625.779376 16.6881648302

Annotations:

YY Y
1627.065003 14.0375950336
1627.844907 5.1737016439
1638.71997 1.385406971

Annotations:

^{3,5}A_{GlcNAc}Y
1665.840134 4.6036103964

Annotations:

B^{1,3}X_{NeuAc} ^{1,5}A_{GlcNAc}Y Z^{0,2}X_{Fuc} Z^{2,5}X_{Gal} Z^{2,5}X_{Man} ^{2,5}X_{Man}Z
1709.790729 9.9005689621

Annotations:

B
1713.759968 1.1952427626

Annotations:

Y
1727.799103 4.1347228289

Annotations:

C
1728.725593 6.1200385094
1786.817061 5.8691864014
1787.859492 22.2040214539

Annotations:

Y
1788.869144 12.7207015753
1898.999964 1.0033167601

Annotations:

Z

There are 4 similar spectra:

Id Structure Score

3642

0.68

3593

0.65

4147

0.60

4961

0.58
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