N-glycans and O-glycans released from purified mammalian glycoprotein standards and proteins from cell lysate

PubMed Entry

Peak Intensity
290.0389481 4.1100269556

Annotations:

B
350.6597413 2.5637987852

Annotations:

^{3,5}X_{GlcNAc}Y B^{1,4}X_{NeuAc} B^{1,5}X_{Man}Y BY^{1,5}X_{Man} B^{2,4}X_{NeuAc} CZ^{1,5}X_{Man} C^{1,5}X_{Man}Z
380.1112175 12.3180930614

Annotations:

CZ^{0,3}X_{Gal} CZ^{1,4}X_{Gal} C^{0,3}X_{Man}Z ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{0,3}A_{GlcNAc}Z BY^{1,4}X_{Man} BY^{0,3}X_{Gal} BY^{1,4}X_{Gal} B^{0,3}X_{Man}Y B^{1,4}X_{Man} CZ^{1,4}X_{Man} C^{0,3}X_{Gal}Z CZ^{0,3}X_{Man} B^{0,3}X_{Gal}Y BY^{0,3}X_{Man} ^{0,3}A_{Gal} ^{1,5}X_{Man}Y Y^{1,5}X_{Man}
455.319996 1.0585825443

Annotations:

YYY
526.3226002 4.7580029964

Annotations:

B
595.2399928 1.0462241173

Annotations:

B^{1,3}X_{Gal} B^{2,4}X_{Gal} ^{1,5}A_{GlcNAc}ZZ ^{1,5}A_{GlcNAc}ZZ
600.2618548 5.9589962959

Annotations:

^{2,4}A_{GlcNAc}Y ^{0,3}A_{GlcNAc}YY ^{3,5}A_{GlcNAc}YY ^{3,5}A_{GlcNAc}YY ^{3,5}A_{Man} YY^{3,5}X_{Fuc} ^{3,5}X_{Gal}YY YY^{3,5}X_{Fuc} Y^{3,5}X_{Gal}Y YY^{3,5}X_{Fuc} Y^{3,5}X_{Man}Y ^{3,5}X_{Man}YY Y^{3,5}X_{Fuc}
655.2531719 55.3591920137

Annotations:

B
656.3655413 16.409817338

Annotations:

^{1,4}A_{GlcNAc}Y ^{3,5}X_{Man}YZ ^{3,5}X_{Man}ZY ^{3,5}X_{Man}Z BY^{2,5}X_{Gal} BY^{2,5}X_{Man} B^{2,5}X_{Gal} B^{2,5}X_{Man}Y CZ^{2,5}X_{Gal} CZ^{2,5}X_{Man} C^{2,5}X_{Man}Z ^{1,5}X_{NeuAc}Z ^{3,5}A_{GlcNAc}YY YZ^{1,4}X_{Fuc} Y^{0,3}X_{Man}Z ZY^{1,4}X_{Fuc} Z^{0,3}X_{Man}Y Z^{1,4}X_{Man} ^{0,3}X_{Gal}Z ^{1,4}X_{Gal}YZ Z^{1,4}X_{Fuc} Z^{0,3}X_{Gal} Z^{1,4}X_{Gal} Z^{0,3}X_{Man} ^{0,3}X_{Man}Z ^{1,4}X_{Gal}ZY ^{0,3}X_{Man}YZ ^{0,3}X_{Man}ZY
680.8950734 3.047927022

Annotations:

CY CY
690.8546883 3.0239334106

Annotations:

^{3,5}A_{GlcNAc}ZZ ^{0,4}X_{Man}ZZ ^{2,4}X_{Man}ZZ ^{0,4}X_{Gal}ZZ Z^{0,4}X_{Gal}Z Z^{1,3}X_{Gal}Z Z^{2,4}X_{Gal}Z ZZ^{0,4}X_{Fuc} Z^{0,4}X_{Man}Z Z^{2,4}X_{Man}Z ^{0,4}X_{GlcNAc}ZZ ^{0,4}X_{GlcNAc}ZZ
692.8578875 4.0172128677

Annotations:

ZZ BZZ BZZ YZ ZY
701.8197659 24.5383226871

Annotations:

Z
702.3812116 11.4552983046

Annotations:

YY
711.0764943 5.1947170496

Annotations:

YY Y BYZ BZY BYZ BZY CZZ CZZ
715.3599902 1.1336414814

Annotations:

YZ ZY
745.3776464 3.1547080278

Annotations:

^{1,4}A_{Man} C^{3,5}X_{Man}Y C^{3,5}X_{Man}Y C^{1,4}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{1,5}X_{Gal} ^{0,4}X_{Man}Y ^{2,4}X_{Man}Y ^{0,4}X_{Man} ^{1,5}X_{Gal}Y ^{0,4}X_{GlcNAc}Y ^{0,3}A_{GlcNAc}YY ^{0,3}A_{GlcNAc}Y ^{3,5}A_{GlcNAc}YY ^{0,2}X_{NeuAc}Y
748.3830344 2.3830192089

Annotations:

^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}Y C^{0,2}X_{Man}Y CY^{0,2}X_{Gal} CY^{0,2}X_{Man} C^{0,2}X_{Gal}Y
774.3782951 7.9141359329

Annotations:

YZ ZY
775.423816 3.1514998674

Annotations:

Y
776.3599888 1.0708976984

Annotations:

C^{3,5}X_{Man}Z B^{3,5}X_{Gal}Y BY^{3,5}X_{Gal} BY^{3,5}X_{Man} B^{3,5}X_{Man}Y C^{0,2}X_{NeuAc}Y C^{3,5}X_{Gal}Z CZ^{3,5}X_{Gal} CZ^{3,5}X_{Man} ^{2,5}A_{GlcNAc}YY ^{2,5}A_{GlcNAc}YY ^{1,3}X_{GlcNAc}YZ ^{1,3}X_{GlcNAc}ZY ^{1,3}X_{GlcNAc}YZ ^{1,3}X_{GlcNAc}ZY ^{1,3}X_{GlcNAc}YZ ^{1,3}X_{GlcNAc}ZY ^{1,3}X_{GlcNAc}YZ ^{1,3}X_{GlcNAc}ZY
782.9864571 40.1442942619

Annotations:

YY
815.0222629 3.2816615105

Annotations:

^{2,5}X_{Man}ZZ ^{2,5}X_{Man}ZZ ^{0,2}A_{GlcNAc}ZZ ^{2,5}A_{Man}YZ ^{2,5}A_{Man}ZY Z^{1,3}X_{Man}Z ^{1,3}X_{Man}ZZ ^{0,4}X_{GlcNAc}ZZ ZZ^{0,4}X_{Fuc} ZZ^{0,4}X_{Fuc}
817.4430643 60.362798214

Annotations:

B
835.4001999 98.6609416008

Annotations:

C
836.4276898 19.8570692539

Annotations:

^{0,4}X_{Man}ZZ ^{2,4}X_{Man}ZZ ^{1,3}X_{Gal}ZZ ^{2,4}X_{Gal}ZZ ^{0,4}X_{Man}ZZ ^{2,4}X_{Man}ZZ ^{0,4}X_{GlcNAc}ZZ
854.8996082 2.7739260197

Annotations:

^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{1,3}X_{Gal}YY ^{2,4}X_{Gal}YY ^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y BZZ BZZ BZZ BZZ ^{0,2}X_{NeuAc}Y ^{2,5}X_{NeuAc}Y Z^{3,5}X_{Fuc}
856.399987 1.0353348255

Annotations:

Y
867.1006794 10.427654624

Annotations:

ZZ
875.3599866 1.1197755337

Annotations:

YZZ ZYZ ZZY YZ ZY
875.9664363 14.1824404001

Annotations:

YZ ZY
894.0634961 3.3661051989
907.3920682 5.4537174702

Annotations:

C^{1,4}X_{Gal}Y C^{0,3}X_{Man}Y ^{1,4}A_{Man} ^{2,4}X_{NeuAc}Y ^{1,3}X_{GlcNAc}Y ^{1,3}X_{GlcNAc}Y
915.5430502 2.8714590073

Annotations:

^{2,4}A_{GlcNAc}ZZ ^{2,4}A_{GlcNAc}ZZ ^{2,4}A_{GlcNAc}ZZ ^{2,4}A_{GlcNAc}ZZ BZ^{0,2}X_{Gal} BZ^{0,2}X_{Man} B^{0,2}X_{Man}Z BZ^{0,2}X_{Gal} BZ^{0,2}X_{Man} B^{0,2}X_{Man}Z B^{0,2}X_{Gal}Z B^{0,2}X_{Gal}Z ^{2,4}X_{NeuAc}Y ^{1,3}X_{GlcNAc}Y ^{1,3}X_{GlcNAc}Y
957.0290038 17.5313256979

Annotations:

Z
985.4567706 4.452772975
997.1108537 6.625608325

Annotations:

CY
1040.497894 3.6217366457

Annotations:

B^{2,4}X_{NeuAc}Y C^{2,4}X_{NeuAc}Z ^{3,5}X_{Gal}YY YY^{3,5}X_{Fuc} Y^{3,5}X_{Gal}Y YY^{3,5}X_{Fuc} Y^{3,5}X_{Man}Y ^{3,5}X_{Man}YY
1095.59062 5.4729808569

Annotations:

^{1,5}A_{Man}Z ^{2,4}A_{GlcNAc}YZ ^{2,4}A_{GlcNAc}ZY ^{2,4}A_{GlcNAc}Z ^{2,4}A_{GlcNAc}YZ ^{2,4}A_{GlcNAc}ZY ^{2,4}A_{GlcNAc}Z BY^{0,2}X_{Gal} BY^{0,2}X_{Man} B^{0,2}X_{Gal} B^{0,2}X_{Man}Y C^{0,2}X_{Man}Z CZ^{0,2}X_{Gal} CZ^{0,2}X_{Man}
1201.553507 21.7727906704

Annotations:

^{2,4}A_{GlcNAc}Y
1202.486143 9.0969474316

Annotations:

YY^{3,5}X_{Fuc} ^{3,5}X_{Gal}YY YY^{3,5}X_{Fuc} Y^{3,5}X_{Gal}Y YY^{3,5}X_{Fuc} Y^{3,5}X_{Man}Y ^{3,5}X_{Man}YY Y^{3,5}X_{Fuc}
1326.629516 2.431764245

Annotations:

BZ BZ
1328.629561 6.8785659075

Annotations:

C^{1,5}X_{NeuAc}Z B^{1,5}X_{NeuAc}Y ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{1,4}A_{GlcNAc}Z CZ^{0,3}X_{Gal} CZ^{1,4}X_{Gal} C^{1,4}X_{Gal}Z C^{0,3}X_{Man}Z BY^{0,3}X_{Gal} BY^{1,4}X_{Gal} BY^{0,3}X_{Man} B^{0,3}X_{Gal} B^{1,4}X_{Gal}Y B^{0,3}X_{Man}Y CZ^{0,3}X_{Man} ^{0,2}X_{NeuAc}YZ ^{0,2}X_{NeuAc}ZY ^{2,5}X_{NeuAc}Z ZZ^{3,5}X_{Fuc}
1344.567922 23.5525081158

Annotations:

CZ BY BY CZ
1362.698321 6.7652157545

Annotations:

CY CY
1363.581975 2.5489698648
1386.659911 24.5861397982

Annotations:

ZZ
1387.760124 4.8216644526

Annotations:

YZ ZY
1466.759973 1.1453968287

Annotations:

^{2,4}X_{NeuAc}YY ^{1,3}X_{GlcNAc}YY ^{1,3}X_{GlcNAc}YY
1482.700408 2.9830679893
1506.719972 1.0898762941

Annotations:

B
1524.683753 43.7699853182

Annotations:

C
1525.771459 22.6847218275
1529.833415 6.228125453

Annotations:

BZ
1547.959972 1.013354063

Annotations:

BY CZ
1548.639972 1.2505807877
1565.874767 6.3498493433

Annotations:

CY
1566.876415 4.4637736082

Annotations:

^{2,4}A_{GlcNAc}
1609.798916 6.9850723743

Annotations:

Y^{0,2}X_{Fuc} ^{2,5}X_{Man}Y ^{2,5}X_{Man}Y
1625.960617 6.0948524475

Annotations:

YY Y
1626.938464 2.8540978432

Annotations:

^{0,2}A_{GlcNAc} C^{2,4}X_{NeuAc} C^{1,3}X_{GlcNAc}
1627.99997 1.1181602478
1668.839969 1.0003786087

Annotations:

^{2,5}A_{GlcNAc}Y ^{1,3}X_{GlcNAc}Z ^{1,3}X_{GlcNAc}Z
1709.863409 35.6441701651

Annotations:

B
1710.746561 18.0838706493

Annotations:

^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{1,3}X_{Gal}YY ^{2,4}X_{Gal}YY ^{0,4}X_{Man}YY ^{2,4}X_{Man}YY ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y
1727.834207 48.2043802738

Annotations:

C
1728.892354 11.1134011745
1787.896498 2.8091917038

Annotations:

Y
1839.759965 1.043872714

There are 4 similar spectra:

Id Structure Score

3643

0.68

4147

0.66

3593

0.65

4961

0.55
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