N-glycans and O-glycans released from purified mammalian glycoprotein standards and proteins from cell lysate

PubMed Entry

Peak Intensity
262.1397984 14.2076034546

Annotations:

B
290.1975573 8.726151228

Annotations:

^{2,4}X_{Fuc}Z B^{3,5}X_{GlcNAc} C^{3,5}X_{Man}Z ^{1,3}X_{Fuc}Z B^{3,5}X_{Gal} B^{3,5}X_{Man}Y ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{3,5}A_{GlcNAc}YZ ^{3,5}A_{GlcNAc}ZY ^{3,5}A_{GlcNAc}Z
304.1382537 13.5692493916

Annotations:

B^{0,4}X_{Gal} B^{1,3}X_{Gal} B^{2,4}X_{Gal} ^{0,2}A_{GlcNAc}YZ B^{0,4}X_{Man}Y CZ^{0,2}X_{GlcNAc} CZ^{0,2}X_{Man} C^{0,4}X_{Man}Z ^{2,5}X_{Fuc}ZZ ^{0,2}A_{GlcNAc}ZY BY^{0,2}X_{GlcNAc} BY^{0,2}X_{Man} B^{0,4}X_{GlcNAc} B^{2,4}X_{GlcNAc} ^{0,2}A_{Man}ZZ ^{0,2}A_{GlcNAc}ZZ BZ^{1,3}X_{Man} B^{1,3}X_{Man}Z BZ^{1,3}X_{GlcNAc} BZ^{1,3}X_{Man} B^{0,4}X_{Man}Z B^{2,4}X_{Man}Z B^{0,4}X_{Gal}Z B^{1,3}X_{Gal}Z B^{2,4}X_{Gal}Z BZ^{0,4}X_{GlcNAc} BZ^{2,4}X_{GlcNAc} BZ^{0,4}X_{Man} BZ^{2,4}X_{Man} B^{0,4}X_{Man}Z B^{2,4}X_{Man}Z
350.2254013 20.5297467709

Annotations:

Z
364.1199966 1.1727911234

Annotations:

B B
368.1579507 13.4071444273

Annotations:

Y
389.1194727 17.5519655943

Annotations:

ZZ
424.1587094 15.1076773405

Annotations:

BY CZ
454.2007323 6.2235051394

Annotations:

CY CYY CY CY
490.1753007 3.7816512585

Annotations:

BZZ BZZ BZZ ^{0,3}A_{GlcNAc}YY ^{0,3}A_{GlcNAc}YY ^{0,3}A_{GlcNAc}Y ^{1,4}A_{GlcNAc}Y C^{0,3}X_{Man}Y C^{1,4}X_{Man} CY^{1,4}X_{GlcNAc} C^{0,3}X_{GlcNAc} C^{0,3}X_{Gal}Y C^{1,4}X_{Gal}Y C^{0,3}X_{Man}Y C^{0,3}X_{Man} C^{0,3}X_{Gal} C^{1,4}X_{Gal} C^{0,3}X_{Man} ^{3,5}X_{Fuc}YZ ^{3,5}X_{Fuc}ZY
508.3660027 8.9638745785

Annotations:

BYZ BZY BYZ BZY BYZ BZY BZ CZZ CZZ CZZ
512.3558852 7.0522711277

Annotations:

^{1,5}A_{GlcNAc}Y ^{1,5}A_{Man}
526.3747389 15.0894551277

Annotations:

B B
547.6989441 4.2612628937

Annotations:

ZY YZ ZYY YYZ YZY ZY YZ
548.3089133 10.2655428648

Annotations:

YY Y
554.3003097 2.4061332941

Annotations:

C^{1,5}X_{Gal}Z B^{1,5}X_{Man}Y BY^{1,5}X_{GlcNAc} B^{1,5}X_{Gal}Y BY^{1,5}X_{Man} B^{1,5}X_{Man} CZ^{1,5}X_{Man} C^{1,5}X_{Man}Z CZ^{1,5}X_{GlcNAc} CZ^{1,5}X_{Man} BY^{1,5}X_{Man} ^{0,3}A_{GlcNAc}Z ^{0,3}A_{GlcNAc}YZ ^{0,3}A_{GlcNAc}ZY ^{0,3}A_{GlcNAc}Z ^{3,5}A_{GlcNAc}ZY ^{3,5}A_{GlcNAc}YZ
556.2302829 5.7314558029

Annotations:

YY YYY YY
571.3402158 2.1635963917

Annotations:

Y
598.8399913 1.0797001123

Annotations:

^{2,5}A_{GlcNAc}YY Z^{0,4}X_{GlcNAc}Y Z^{1,3}X_{GlcNAc}Y ^{1,3}X_{GlcNAc}ZY Z^{0,4}X_{Man}Y Y^{0,4}X_{Man}Z Z^{1,3}X_{Man} Z^{2,4}X_{Man}Y Y^{2,4}X_{Man}Z ^{0,4}X_{Fuc}Z Z^{0,4}X_{Gal}Y Y^{0,4}X_{Gal}Z Z^{1,3}X_{Gal}Y Y^{1,3}X_{Gal}Z Z^{2,4}X_{Gal}Y Y^{2,4}X_{Gal}Z ZY^{1,3}X_{GlcNAc} ^{0,4}X_{Fuc}YZ YZ^{1,3}X_{GlcNAc} ^{0,4}X_{Fuc}ZY ^{0,4}X_{Fuc}Z Z^{0,4}X_{Man}Y Y^{0,4}X_{Man}Z Z^{2,4}X_{Man}Y Y^{2,4}X_{Man}Z Y^{0,4}X_{GlcNAc}Z Y^{1,3}X_{GlcNAc}Z ^{0,4}X_{GlcNAc}Z ^{1,3}X_{GlcNAc}YZ ^{1,5}X_{Man}
616.4197669 2.1096096039

Annotations:

^{0,3}A_{GlcNAc}YY ^{0,3}A_{GlcNAc}Y ^{1,4}A_{GlcNAc}YY CY^{1,4}X_{Man} C^{1,4}X_{Man}Y CY^{1,4}X_{GlcNAc} C^{0,3}X_{Gal}Y C^{1,4}X_{Gal}Y CY^{1,4}X_{Man} CY^{0,3}X_{GlcNAc} C^{0,3}X_{Man}Y C^{1,4}X_{Man} ^{1,4}A_{Man} CY^{0,3}X_{Man} C^{0,3}X_{Man}Y ^{1,5}A_{GlcNAc}ZZ
619.4666799 9.1973572969

Annotations:

ZZ
628.2983361 2.4640008211

Annotations:

ZY YZ
629.2731579 5.6425597668

Annotations:

Y YY Y
630.1109968 21.3486276865

Annotations:

Y YY Y
649.3223959 9.0940276384

Annotations:

ZY YZ
671.3199897 1.0519503355

Annotations:

Z^{1,3}X_{Man}Y Y^{1,3}X_{Man}Z ZY^{1,3}X_{Man} ^{0,4}X_{Fuc}YZ YZ^{1,3}X_{Man} ^{0,4}X_{Fuc}ZY Y^{0,4}X_{Man}Z Y^{1,3}X_{Man}Z Y^{2,4}X_{Man}Z ^{0,4}X_{GlcNAc}YZ ^{0,4}X_{GlcNAc}ZY ^{0,4}X_{GlcNAc}YZ ^{0,4}X_{GlcNAc}ZY Y^{0,2}X_{GlcNAc} Y^{0,2}X_{Man} ^{0,2}X_{Man}Y Z^{0,4}X_{Man}Y Z^{1,3}X_{Man}Y Z^{2,4}X_{Man}Y ^{2,5}A_{GlcNAc}Y ^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z ^{0,4}X_{Man}Z ^{2,4}X_{Man}Z ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{1,3}X_{GlcNAc} ^{0,4}X_{Man}Z ^{2,4}X_{Man}Z ^{0,4}X_{GlcNAc}Z ^{1,3}X_{GlcNAc}Z ^{1,3}X_{GlcNAc}Z
676.7997946 7.0557094812

Annotations:

Z^{0,3}X_{GlcNAc} ^{1,4}X_{Fuc}Z Z^{0,3}X_{Man} ^{0,3}X_{Man}Z
677.3188508 5.0846271515

Annotations:

Z^{0,3}X_{GlcNAc} ^{1,4}X_{Fuc}Z Z^{0,3}X_{Man} ^{0,3}X_{Man}Z ^{1,5}A_{GlcNAc}Z
689.918966 7.1707812548
701.9130717 9.3382902145

Annotations:

Z
709.3920631 5.8354313374

Annotations:

^{0,4}X_{Fuc}Y Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc} Y^{0,4}X_{Man} Y^{2,4}X_{Man} Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} Y^{0,4}X_{Man} Y^{2,4}X_{Man} Y^{0,4}X_{GlcNAc} ^{0,4}X_{GlcNAc}
710.0542733 9.2680803537

Annotations:

^{3,5}A_{GlcNAc}ZZ ^{3,5}A_{GlcNAc}ZZ
710.8799888 1.2961848974

Annotations:

Y BYZ BZY BZ CZZ
711.582469 2.8476430178

Annotations:

BYZ BZY BZ CZZ
722.5360362 22.9647815228

Annotations:

Z
729.3564376 4.7674514055

Annotations:

BYY BY CYZ CZY CZ
730.3826421 24.0200412273

Annotations:

Z
730.9945529 16.7951447964

Annotations:

Y ZYY YYZ YZY ZYY YYZ YZY
737.3186408 5.5965924263

Annotations:

^{3,5}A_{GlcNAc}
768.4208952 2.506139636

Annotations:

^{2,5}X_{Fuc}
850.3781844 2.7031369209

Annotations:

BY CZ
891.4228238 2.8900494576

Annotations:

BY BYY BY BY CZ CYZ CZY CZ CZ
895.4982263 9.3159866333

Annotations:

YY YY
928.3199839 1.1324342489
935.5408935 2.949587822

Annotations:

^{0,2}X_{Fuc}YZ Y^{2,5}X_{Man}Z ^{0,2}X_{Fuc}ZY Z^{2,5}X_{Man}Y ^{2,5}X_{Man}YZ ^{2,5}X_{Man}ZY
948.5599835 1.204629302
951.4210249 20.0284609795

Annotations:

^{2,4}A_{GlcNAc}Y ^{2,4}A_{GlcNAc}YY ^{2,4}A_{GlcNAc}Y ^{2,4}A_{GlcNAc}Y C^{0,2}X_{Man}Y C^{0,2}X_{GlcNAc} C^{0,2}X_{Man} C^{0,2}X_{Gal}Y C^{0,2}X_{Man}Y C^{0,2}X_{Gal} C^{0,2}X_{Man} ^{2,5}X_{Fuc}YZ ^{2,5}X_{Fuc}ZY
952.4349428 4.9286600351

Annotations:

YYY YY
966.4574886 2.8978186846
994.5017811 3.9479072094

Annotations:

Y^{0,2}X_{Gal}Y ^{0,2}X_{Fuc}YY YY^{0,2}X_{GlcNAc} YY^{0,2}X_{Man} Y^{0,2}X_{Man}Y Y^{0,2}X_{Man}Y ^{2,5}X_{Man}YY ^{0,2}X_{Fuc}Y Y^{2,5}X_{GlcNAc} Y^{2,5}X_{Man}
1036.513287 10.8588012457

Annotations:

Z^{0,4}X_{Man} Z^{0,4}X_{GlcNAc}Y Z^{0,4}X_{GlcNAc}Y Z^{0,4}X_{Gal}Y Y^{0,4}X_{Gal}Z Z^{1,3}X_{Gal}Y Y^{1,3}X_{Gal}Z Z^{2,4}X_{Gal}Y Y^{2,4}X_{Gal}Z ZY^{1,3}X_{Man} ^{0,4}X_{Fuc}YZ YZ^{1,3}X_{Man} ^{0,4}X_{Fuc}ZY ZY^{0,4}X_{GlcNAc} ZY^{2,4}X_{GlcNAc} ZY^{0,4}X_{Man} ZY^{2,4}X_{Man} YZ^{0,4}X_{GlcNAc} YZ^{2,4}X_{GlcNAc} YZ^{0,4}X_{Man} YZ^{2,4}X_{Man} Z^{0,4}X_{Man}Y Y^{0,4}X_{Man}Z Z^{0,4}X_{Man}Y Y^{0,4}X_{Man}Z Z^{1,3}X_{Man} Z^{2,4}X_{Man}Y Y^{2,4}X_{Man}Z Z^{2,4}X_{Man}Y Y^{2,4}X_{Man}Z ^{0,4}X_{Fuc}Z Y^{0,4}X_{GlcNAc}Z Y^{0,4}X_{GlcNAc}Z ^{0,4}X_{GlcNAc}Z ^{0,4}X_{GlcNAc}YZ ^{0,4}X_{GlcNAc}ZY ^{0,4}X_{GlcNAc}Z
1054.642335 4.3483438492

Annotations:

^{0,4}X_{Fuc}YY ^{0,4}X_{Fuc}Y Y^{0,4}X_{Man} Y^{0,4}X_{GlcNAc}Y Y^{0,4}X_{GlcNAc}Y Y^{0,4}X_{Gal}Y Y^{1,3}X_{Gal}Y Y^{2,4}X_{Gal}Y YY^{1,3}X_{Man} YY^{0,4}X_{GlcNAc} YY^{2,4}X_{GlcNAc} YY^{0,4}X_{Man} YY^{2,4}X_{Man} Y^{0,4}X_{Man}Y Y^{0,4}X_{Man}Y Y^{1,3}X_{Man} Y^{2,4}X_{Man}Y Y^{2,4}X_{Man}Y ^{0,4}X_{GlcNAc}Y ^{0,4}X_{GlcNAc}YY ^{0,4}X_{GlcNAc}Y
1056.741316 2.3830187321
1058.439981 1.0148545504
1076.481701 5.0149680376

Annotations:

BZ
1077.500076 2.811044693
1078.999981 1.1791067123
1079.538956 2.878215909
1080.481491 6.3083586693

Annotations:

Z
1096.639344 23.4385834932

Annotations:

ZY YZ ZYY YYZ YZY ZY YZ
1097.495175 6.6203995943
1098.554629 5.8660033941

Annotations:

YY Y
1113.516669 75.2899745703

Annotations:

^{2,4}A_{GlcNAc}Y ^{2,4}A_{GlcNAc}
1114.588122 40.0403549671

Annotations:

YY YYY YY
1140.641227 4.6227064133

Annotations:

C^{1,5}X_{Gal} ^{0,2}X_{Gal}Y Y^{0,2}X_{Man}Y Y^{0,2}X_{GlcNAc} Y^{0,2}X_{Man} ^{0,2}X_{Man}Y ^{0,2}X_{Man}YY ^{0,2}X_{Man}Y
1150.581287 8.019254446

Annotations:

^{3,5}A_{GlcNAc}ZY ^{3,5}A_{GlcNAc}YZ Z^{0,3}X_{Man}Z Z^{0,3}X_{Gal}Z Z^{1,4}X_{Gal}Z ZZ^{1,4}X_{GlcNAc} ^{0,3}X_{Fuc}ZZ Z^{3,5}X_{Man}Z Z^{0,3}X_{Man}Z ^{3,5}X_{Man}ZZ
1155.497788 2.2637341022

Annotations:

^{0,2}A_{GlcNAc}ZY ^{0,2}A_{GlcNAc}YZ B^{1,3}X_{GlcNAc} B^{1,3}X_{GlcNAc}
1240.617583 2.552506566

Annotations:

ZZ
1242.698953 8.0965437889

Annotations:

Z Z
1256.672531 7.999396801

Annotations:

B
1257.601695 6.3407146931

Annotations:

YY^{0,4}X_{GlcNAc} YY^{2,4}X_{GlcNAc} YY^{0,4}X_{Man} YY^{2,4}X_{Man} Y^{0,4}X_{Man}Y Y^{2,4}X_{Man}Y Y^{0,4}X_{GlcNAc}Y ^{0,4}X_{GlcNAc}Y
1258.524275 11.6731249094

Annotations:

ZY YZ
1260.663704 45.890615344

Annotations:

Y YY Y
1261.595338 30.1567255259
1281.607971 25.6490547657

Annotations:

ZZ
1282.559331 14.0948234797
1299.632011 6.3908936977

Annotations:

ZY YZ
1385.814932 2.7983766794

Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{0,4}X_{GlcNAc} Z^{2,4}X_{GlcNAc} Z^{0,4}X_{Man} Z^{2,4}X_{Man} ^{0,4}X_{Man}Z ^{2,4}X_{Man}Z ^{0,4}X_{GlcNAc}Z
1403.799973 1.1083474159

Annotations:

^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc} Y^{0,4}X_{Man} Y^{2,4}X_{Man} ^{0,4}X_{Man}Y ^{2,4}X_{Man}Y ^{0,4}X_{GlcNAc}Y
1428.902021 2.354516983
1445.777531 45.001835227

Annotations:

Z
1446.781896 26.9747750759
1461.902089 2.613505125

Annotations:

Z
1462.741094 4.0469709635

There are 2 similar spectra:

Id Structure Score

3638

0.58

3556

0.57
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