The Glycomic MS Database and Repository

Responsible:

Yolanda H Mthembu . Dept. of Laboratory Medicine, Gothenburg University . yolandamthembu@gmail.com

Sample preparation




if(vLc != null){

HPLC


1. General settings

Instrument: Agilent 1100 Series

Manufacturer url: https://www.agilent.com/home

Injector: NA

Injector settings: NA


2. Column

Manufacturer: In-house

Model: Hypercarb

Type of Chromatography: Reversed phase

Type of Material: Porous graphitized carbon

Column diameter: 0.25

Column length: 100

Particle size: 5

Manufacturer url: NA


3. Method run

Temperature: RT

Solvent a: 10 mM NH4HCO3

Solvent b: 0-40% acetonitrile in 10 mM NH4HCO3

Other solvent:

Flow rate: 0.010 mL/min

Flow gradient:

Run time: Over 40 min

Phase: Reversed Phase

}

MS



1. General features

Date stamp: Tue May 28 00:00:00 CEST 2019

Instrument: Thermo Fisher LTQ Linear Ion Trap ESI

Customizations from the manufacturer's specification:

ion_mode: -


2. Ion sources

(a) Electrospray ionisation - ESI

Sprayer type : Fed

Interface name:

Other data for the Interface:

Sprayer name: ESI

Other data for the sprayer:

Relevant voltages:

Degree of prompt fragmentation evaluated: Yes

In-source dissociation performed: Yes

Other parameters if discriminant for the experiment:


(b) MALDI

Plate composition (or type): NA

Matrix composition: NA

Deposition technique: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: NA

PSD (or LID/ISD) summary: NA

Operation with or without delayed extraction: NA

Laser type: NA

Other laser related parameters: NA

url: NA


3. Ion transfer optics

Hardware options:


3. Post-source componentry

Collision-Induced Dissociation - CID

CID. Gas composition: NA

CID. Gas pressure: NA

CID. Collision energy: NA

Electron Transfer Dissociation - ETD

ETD. Reagent gas: NA

ETD. Pressure: NA

ETD. Reaction time: NA

ETD. Reagent atoms: NA

Electron Capture Dissociation - ECD

ECD. Emitter type: NA

ECD. Voltage: NA

ECD. Current: NA

TOF drift tube

TOF. Reflectron status: NA

Ion Trap

Ion trap. Final MS stage achieved: NA

Ion mobility

Ion mobility. Gas type : NA

Ion mobility. Pressure: NA

Ion mobility. Instrument parameters: NA

FT-ICR

Peak selection: NA

FT-ICR. Pulse: NA

FT-ICR. Width: NA

FT-ICR. IR: Decay time: NA

FT-ICR. IR: NA

FT-ICR. Other parameters: NA

Detectors

Detector type: NA