The Glycomic MS Database and Repository

Responsible:

Chunsheng Jin . Institute of Biomedicine, Gothenburg University . chunsheng.jin@medkem.gu.se

Sample preparation


1.Sample Origin

General information:
Membrane protein samples from mouse leg muscle under different conditions

Growth/harvest conditions for recombinantly produced material:

Treatments and/or storage conditions:

For commercial material, vendor and applicable item information:

Generation of chemically derived material:


2. Sample Processing

Purification steps:


2. Defined Sample

Sample name:



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HPLC


1. General settings

Instrument: Agilent 1100 Series

Manufacturer url: https://www.agilent.com/home

Injector: NA

Injector settings: NA


2. Column

Manufacturer: In-house

Model: Custom made Hypercarb

Type of Chromatography:

Type of Material:

Column diameter:

Column length:

Particle size:

Manufacturer url: NA


3. Method run

Temperature:

Solvent a: 10 mM Ammonium Biocarbonate

Solvent b: 10 mM Ammonium Biocarbonate in 80% AcCN

Other solvent:

Flow rate: 10 ul/min

Flow gradient:

Run time:

Phase: normal phase

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MS



1. General features

Date stamp: Thu Jul 05 00:00:00 CEST 2018

Instrument: Thermo Fisher LTQ Linear Ion Trap ESI

Customizations from the manufacturer's specification:

ion_mode: -


2. Ion sources

(a) Electrospray ionisation - ESI

Sprayer type : fed

Interface name:

Other data for the Interface:

Sprayer name: ESI

Other data for the sprayer:

Relevant voltages:

Degree of prompt fragmentation evaluated: Yes

In-source dissociation performed: Yes

Other parameters if discriminant for the experiment:


(b) MALDI

Plate composition (or type): NA

Matrix composition: NA

Deposition technique: NA

Relevant voltages: NA

Degree of prompt fragmentation evaluated: NA

PSD (or LID/ISD) summary: NA

Operation with or without delayed extraction: NA

Laser type: NA

Other laser related parameters: NA

url: NA


3. Ion transfer optics

Hardware options:


3. Post-source componentry

Collision-Induced Dissociation - CID

CID. Gas composition: Helieum

CID. Gas pressure:

CID. Collision energy: 35%

Electron Transfer Dissociation - ETD

ETD. Reagent gas: NA

ETD. Pressure: NA

ETD. Reaction time: NA

ETD. Reagent atoms: NA

Electron Capture Dissociation - ECD

ECD. Emitter type: NA

ECD. Voltage: NA

ECD. Current: NA

TOF drift tube

TOF. Reflectron status: NA

Ion Trap

Ion trap. Final MS stage achieved: NA

Ion mobility

Ion mobility. Gas type : NA

Ion mobility. Pressure: NA

Ion mobility. Instrument parameters: NA

FT-ICR

Peak selection: NA

FT-ICR. Pulse: NA

FT-ICR. Width: NA

FT-ICR. IR: Decay time: NA

FT-ICR. IR: NA

FT-ICR. Other parameters: NA

Detectors

Detector type: NA